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नवीनतम संदेश 4
2022-06-20 08:34:01
29 voters206 views05:34
2022-06-20 08:33:01
An infant born to a 34-year-old woman has a flat facial profile, prominent epicanthal folds, and a holosystolic murmur heard loudest at the left sternal border. Karyotype analysis is consistent with trisomy 21. Maternal and paternal karyotypes are normal. A restriction fragment length polymorphism (RFLP) analysis is conducted to determine the parental origin of the extra chromosome. DNA samples from the child, mother, and father are obtained and the DNA is fragmented with a restriction enzyme. The fragments are then sorted by size using the Southern blot technique. Labeling is done using a probe that binds to a specific DNA sequence close to the centromere of chromosome 21. RFLP analysis for the child, mother, and father is shown below.
206 views05:33
2022-06-19 20:32:01
Educational objective: In X-linked recessive inheritance 1) affected males will always produce unaffected sons and carrier daughters, and 2) carrier females have a SOo/o chance of producing affected sons and carrier daughters. G6PD deficiency follows this inheritance pattern and causes acute hemolytic anemia in response to oxidant drugs.
87 views17:32
2022-06-19 08:32:01
20 voters189 views05:32
2022-06-19 08:32:01
180 views05:32
2022-06-18 20:31:02
Educational objective: Southern blotting is a technique used to identify DNA mutations. It involves restriction endonuclease digestion of sample DNA, gel electrophoresis, and gene identification with a labeled DNA probe.
223 views17:31
2022-06-18 08:31:08
41 views05:31
2022-06-18 08:31:01
5 voters43 views05:31
2022-06-17 20:31:31
Educational objective: All 3 prokaryotic DNA polymerases can remove mismatched nucleotides via their 3' to 5' exonuclease ("proofreading") activity. Only DNA polymerase I has 5' to 3' exonuclease activity, which is used to remove the RNA primer synthesized by RNA primase.
123 views17:31
2022-06-17 08:30:43
29 voters220 views05:30
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